Coding

Part:BBa_K4169020

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)


FrmB is a promiscuous serine hydrolase; its highest specific activity is with the substrate S-formyl

The Escherichia coli genes frmB encode two uncharacterized proteins, it had the highest hydrolytic activity toward S-formylglutathione, an intermediate of the glutathione-dependent pathway of formaldehyde detoxification.It is a hydrolase that assists frmA to complete formaldehyde dehydrogenation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None